Gateway lr clonase ii manual

specifically designed for MultiSite Gateway® and MultiSite Gateway® Pro. Gateway® LR Clonase™ II Plus enzyme mix contains the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), and the E. coli-encoded protein Integration Host Factor (IHF) (1), and reaction buffer provided in a single mix for convenient reaction set up. Gateway® LR Clonase™ II Plus. Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone. Gateway® LR Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps. The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°www.doorway.ru Size: 1MB.

Gateway® LR Clonase™ II Plus Enzyme Mix 40 µl µl 2 µg/µl Proteinase K Solution 40 µl µl Storage Store Gateway® LR Clonase™ II Plus at °C (in a non-frost-free freezer) for up to 6 months. For long term storage, store at °C Part no. pps Rev. date: 11 July Page 4 of 4 Next Steps. Note: LR Clonase II (no Plus) is a different enzyme, to be used for "classic" (non-multisite) Gateway reactions. Note: make sure to store LR Clonase II Plus at degrees, as it seems to be especially labile. (We usually also. Thaw on ice the Invitrogen LR Clonase II enzyme mix for about 2 minutes. Online Library Gateway Technology Clonase Ii Manual Gateway Technology Clonase Ii Manual Right here, we have countless ebook gateway technology clonase ii manual and collections to check out. We additionally have enough money variant types and plus type of the books to browse. The within acceptable limits book, fiction, history, novel.

Gateway Lr Clonase Ii Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/, based on 1 PubMed citations. The LR clonase cloning step is very easy and efficient, requiring little time or 2. Clone PCR product into entry vector (CGT) using traditional. and 2 μL of Gateway LR Clonase II enzyme mix. The reaction conditions and Proteinase K treatment were according to the manufacturer's instructions (10). the.